Direct conversion of glucose to malate by synthetic metabolic engineering.

@article{Ye2013DirectCO,
  title={Direct conversion of glucose to malate by synthetic metabolic engineering.},
  author={Xiaoting Ye and K. Honda and Y. Morimoto and K. Okano and H. Ohtake},
  journal={Journal of biotechnology},
  year={2013},
  volume={164 1},
  pages={
          34-40
        }
}
  • Xiaoting Ye, K. Honda, +2 authors H. Ohtake
  • Published 2013
  • Medicine, Chemistry
  • Journal of biotechnology
  • Synthetic metabolic engineering enables us to construct an in vitro artificial synthetic pathways specialized for chemical manufacturing through the simple heat-treatment of the recombinant mesophiles having thermophilic enzymes, followed by rational combination of those biocatalytic modules. In this work, we constructed a synthetic pathway capable of direct conversion of glucose to malate. The reversible carboxylation of pyruvate catalyzed by a malic enzyme derived from Thermococcus… CONTINUE READING

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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 34 REFERENCES
    Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway
    66
    Biofuel production by in vitro synthetic enzymatic pathway biotransformation.
    67