Direct contact with stroma inhibits proliferation of human long-term culture initiating cells.

@article{Verfaillie1996DirectCW,
  title={Direct contact with stroma inhibits proliferation of human long-term culture initiating cells.},
  author={Catherine M. Verfaillie and P Catanzaro},
  journal={Leukemia},
  year={1996},
  volume={10 3},
  pages={498-504}
}
Significantly less long-term culture initiating cells (LTC-IC) are recovered from cultures in which progenitors are cultured in contact with stroma (stroma-contact) than when cultured separated from stroma by a transwell (stroma-noncontact). This suggests that direct contact with stroma inhibits either proliferation or survival of LTC-IC. Using the membrane intercallating fluorochrome, PKH-26, we demonstrated that significantly less LTC-IC plated for 14 days in stroma-contact cultures… CONTINUE READING