Direct chemical method for sequencing RNA.

  title={Direct chemical method for sequencing RNA.},
  author={Debra A. Peattie},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={76 4},
  • Debra A. Peattie
  • Published 1979 in
    Proceedings of the National Academy of Sciences…
Four different base-specific chemical reactions generate a means of directly sequencing RNA terminally labeled with 32P. After a partial, specific modification of each kind of RNA base, an amine-catalyzed strand scission generates labeled fragments whose lengths determine the position of each nucleotide in the sequence. Dimethyl sulfate modifies guanosine. Diethyl pyrocarbonate attacks primarily adenosine. Hydrazine attacks uridine and cytidine, but salt suppresses the reaction with uridine. In… CONTINUE READING
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