Direct charging of tRNACUA with pyrrolysine in vitro and in vivo

  title={Direct charging of tRNACUA with pyrrolysine in vitro and in vivo},
  author={Sherry Kathleen Blight and Ross C. Larue and Anirban Mahapatra and David Gordon Longstaff and Edward K. Chang and Gang Zhao and Patrick T. Kang and Kari B. Green-Church and Michael K. Chan and Joseph Adrian Krzycki},
Pyrrolysine is the 22nd amino acid. An unresolved question has been how this atypical genetically encoded residue is inserted into proteins, because all previously described naturally occurring aminoacyl-tRNA synthetases are specific for one of the 20 universally distributed amino acids. Here we establish that synthetic l-pyrrolysine is attached as a free molecule to tRNACUA by PylS, an archaeal class II aminoacyl-tRNA synthetase. PylS activates pyrrolysine with ATP and ligates pyrrolysine to… CONTINUE READING
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