Direct biochemical evidence for type III secretion-dependent translocation of the AvrBs2 effector protein into plant cells.

Abstract

The calmodulin-dependent adenylate cyclase domain (Cya) of the Bordetella pertussis cyclolysin was used as a reporter protein to study the direct translocation of the Xanthomonas effector protein, AvrBs2, into the plant host cell. Adenylate cyclase activity (production of cAMP) depends on the presence of eukaryotic plant calmodulin and is only active after… (More)

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