Direct binding to ceramide activates protein kinase Czeta before the formation of a pro-apoptotic complex with PAR-4 in differentiating stem cells.

@article{Wang2005DirectBT,
  title={Direct binding to ceramide activates protein kinase Czeta before the formation of a pro-apoptotic complex with PAR-4 in differentiating stem cells.},
  author={Guanghu Wang and Jeane M. Silva and Kannan Krishnamurthy and Eric Tran and Brian Gene Condie and Erhard Bieberich},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 28},
  pages={26415-24}
}
We have reported that ceramide mediates binding of atypical protein kinase C (PKC) zeta to its inhibitor protein, PAR-4 (prostate apoptosis response-4), thereby inducing apoptosis in differentiating embryonic stem cells. Using a novel method of lipid vesicle-mediated affinity chromatography, we showed here that endogenous ceramide binds directly to the PKCzeta.PAR-4 complex. Ceramide and its analogs activated PKCzeta prior to binding to PAR-4, as determined by increased levels of phosphorylated… CONTINUE READING

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