Direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate with a centrifugal analyzer.

@article{Hansen1978DirectAO,
  title={Direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate with a centrifugal analyzer.},
  author={J. Lundsgaard Hansen and E F Freier},
  journal={Clinical chemistry},
  year={1978},
  volume={24 3},
  pages={475-9}
}
Methods are described for direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate in plasma with the GEMSAEC centrifugal analyzer. The methods for lactate, beta-hydroxybutyrate, and acetoacetate are kinetic and ratiometric, eliminating the need for specimen-blank assays. The pyruvate method is an end-point assay, because endogenous lactate dehydrogenase interferes in a kinetic pyruvate assay. The methods are precise and accurate and 1-min of analysis time is adequate for each… CONTINUE READING
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Clinical and Biochemical Aspects of Lactic Acidosis

  • R. D. Cohen, H. F. Woods
  • Blackwell Scientific Pubs.,
  • 1976
1 Excerpt

Electrolytes. In Fundamentals of Clinical Chemistry, 2nd

  • N. W. Tietz
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