Direct alteration of erythrocyte membrane properties by 1-chloro-2,4-dinitrobenzene without oxidant challenge.

@article{Chiu1993DirectAO,
  title={Direct alteration of erythrocyte membrane properties by 1-chloro-2,4-dinitrobenzene without oxidant challenge.},
  author={D T Y Chiu and Ka Man Lai and Chunhua Xu and Sean S. Liour and Jong-Bum Lee and Tsan Zon Liu},
  journal={Experimental hematology},
  year={1993},
  volume={21 1},
  pages={114-8}
}
The electrophilic agent, 1-chloro-2,4-dinitrobenzene (CDNB), has been widely used as an intracellular glutathione-depleting agent. However, its possible effect on the functional integrity of cell membrane has largely been neglected. Incubation of human erythrocytes (RBC) with various concentrations of CDNB (0.5 to 5.0 mM) in potassium-free, phosphate buffered saline containing ouabain resulted in a drastic depletion of cellular glutathione as well as a dose-dependent increase in passive… CONTINUE READING