Direct activation of fission yeast adenylate cyclase by the Gpa2 Galpha of the glucose signaling pathway.

@article{Ivey2005DirectAO,
  title={Direct activation of fission yeast adenylate cyclase by the Gpa2 Galpha of the glucose signaling pathway.},
  author={Frank Douglas Ivey and Charles S Hoffman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 17},
  pages={
          6108-13
        }
}
G protein-mediated signaling is implicated in yeast and fungal cAMP pathways. By two-hybrid screens and pull-down experiments, we show that the fission yeast Gpa2 Galpha binds an N-terminal domain of adenylate cyclase, comprising a moderately conserved sequence within a region otherwise poorly related to other fungal adenylate cyclases. Overexpressing this domain in yeast perturbs cAMP signaling, which is restored by Gpa2 coexpression. Mutations affecting this domain, over 1,100 residues from… CONTINUE READING

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