Direct Transfer and Expression of Human GM-CSF in Tobacco Suspension Cell using Agrobacterium-Mediated Transfer System

@article{Kim2004DirectTA,
  title={Direct Transfer and Expression of Human GM-CSF in Tobacco Suspension Cell using Agrobacterium-Mediated Transfer System},
  author={Young Han Kim and Tae Ho Kwon and Yang Moon Sik},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={78},
  pages={133-138}
}
Direct gene transfer methods were established for cell suspension cultures of tobacco (Nicotiana tabacum cv. Havana) using Agrobacterium-mediated transformation system. Agrobacterium strain LBA4404 containing a binary vector carrying the hGM-CSF gene was introduced into tobacco suspension cells. Cell culture cycle affected transformation efficiency. The cells at 5 days after subculture showed the highest response growing 9.7 transformed cells per plate. Genomic PCR and northern blot analysis… CONTINUE READING

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