Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies.

@article{Chaturvedi2000DirectPO,
  title={Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies.},
  author={Sudha Chaturvedi and B Rodeghier and Jian-yong Fan and Carol M. McClelland and Brian L. Wickes and Vishnu Chaturvedi},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 5},
  pages={2007-9}
}
Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry. 

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Cryptococcus neoformans

A. Casadevall, J. R. Perfect
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