Direct PCR detection of Escherichia coli O157:H7.

@article{FodeVaughan2003DirectPD,
  title={Direct PCR detection of Escherichia coli O157:H7.},
  author={K A Fode-Vaughan and James S. Maki and Jesse Benson and M. L. Collins},
  journal={Letters in applied microbiology},
  year={2003},
  volume={37 3},
  pages={239-43}
}
AIMS This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC). METHODS AND RESULTS Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk. CONCLUSIONS PCR reactions containing one cell yielded a DPCR product. SIGNIFICANCE… CONTINUE READING
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