Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach

@article{Nakamura2009DirectMD,
  title={Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach},
  author={Shota Nakamura and C Yang and Naomi Sakon and Mayo Ueda and Takahiro Tougan and Akifumi Yamashita and Naohisa Goto and Kazuo Takahashi and Teruo Yasunaga and Kazuyoshi Ikuta and Tetsuya Mizutani and Yoshiko Okamoto and Michihira Tagami and Ryoji Morita and N. Maeda and Jun Kawai and Yoshihide Hayashizaki and Yoshiyuki Nagai and Toshihiro Horii and Tetsuya Iida and Takaaki Nakaya},
  journal={PLoS ONE},
  year={2009},
  volume={4},
  pages={e924 - 2364}
}
With the severe acute respiratory syndrome epidemic of 2003 and renewed attention on avian influenza viral pandemics, new surveillance systems are needed for the earlier detection of emerging infectious diseases. We applied a "next-generation" parallel sequencing platform for viral detection in nasopharyngeal and fecal samples collected during seasonal influenza virus (Flu) infections and norovirus outbreaks from 2005 to 2007 in Osaka, Japan. Random RT-PCR was performed to amplify RNA extracted… CONTINUE READING
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