Direct Measurement of the Ionization State of an Essential Guanine in the Hairpin Ribozyme

  title={Direct Measurement of the Ionization State of an Essential Guanine in the Hairpin Ribozyme},
  author={Lu Feng Liu and Joseph W. Cottrell and Lincoln Greyson Scott and M. J. Fedor},
  booktitle={Nature chemical biology},
Active site guanines are critical for self-cleavage reactions of several ribozymes, but their precise functions in catalysis are unclear. To learn whether protonated or deprotonated forms of guanine predominate in the active site, microscopic pKa values were determined for ionization of 8-azaguanosine substituted for G8 in the active site of a fully functional hairpin ribozyme in order to determine microscopic pKa values for 8-azaguanine deprotonation from the pH dependence of fluorescence… CONTINUE READING

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