Direct MS/MS analysis of proteins blotted on membranes by a matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer.

@article{Nakanishi2005DirectMA,
  title={Direct MS/MS analysis of proteins blotted on membranes by a matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer.},
  author={Tsuyoshi Nakanishi and Iwao Ohtsu and Masaru Furuta and Eiji Ando and Osamu Nishimura},
  journal={Journal of proteome research},
  year={2005},
  volume={4 3},
  pages={743-7}
}
We constructed a system for the microscale identification of membrane-blotted proteins by proteolytic digestion using an instrument developed with piezoelectric chemical inkjet technology and MS/MS analyses of the resulting peptides with a matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer (MALDI-QIT-TOF MS). Using this system, bovine serum albumin was clearly identified at levels less than 100 fmol, and proteins from an Escherichia coli… CONTINUE READING

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