Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts.

  title={Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts.},
  author={Caroline Kubaczka and Claire E. Senner and Monika Cierlitza and Marcos Jes{\'u}s Ara{\'u}zo-Bravo and Peter Kuckenberg and Michael Peitz and Myriam Hemberger and Hubert Schorle},
  journal={Cell stem cell},
  volume={17 5},
Trophoblast stem cells (TSCs) arise from the first cell fate decision in the developing embryo and generate extra-embryonic lineages, giving rise to the fetal portion of the placenta. Mouse embryonic and extra-embryonic lineages are strictly separated by a distinct epigenetic barrier, which is not fully overcome following expression of TSC-determining factors in embryonic stem cells. Here, we show that transient expression of Tfap2c, Gata3, Eomes, and Ets2 is sufficient to reprogram mouse… CONTINUE READING