Transport of H(+), Na(+) and K(+) across the posterior midgut of blood-fed mosquitoes (Aedes aegypti).
The alkaline neocuproine-copper reagent recommended by Dygert et ~2. for the determination of reducing sugars (1) can be used for the direct assay of uric acid in feces of uricogenic animals. The reaction requires two stable solutions. Solution I contains 40 g sodium carbonate, 16 g glycine, and 0.5 g cupric sulphate/liter. Solution II contains 0.5 g neocuproine hydrochloride/100 ml. The reagent is a mixture of 10 vol of solution I and 1 vol of solution II. This mixture yellows on standing and should be discarded after a few hours.