Direct Comparisons of Illumina vs. Roche 454 Sequencing Technologies on the Same Microbial Community DNA Sample

@inproceedings{Luo2012DirectCO,
  title={Direct Comparisons of Illumina vs. Roche 454 Sequencing Technologies on the Same Microbial Community DNA Sample},
  author={Chengwei Luo and Despina Tsementzi and Nikos C Kyrpides and Timothy Read and Konstantinos T. Konstantinidis},
  booktitle={PloS one},
  year={2012}
}
Next-generation sequencing (NGS) is commonly used in metagenomic studies of complex microbial communities but whether or not different NGS platforms recover the same diversity from a sample and their assembled sequences are of comparable quality remain unclear. We compared the two most frequently used platforms, the Roche 454 FLX Titanium and the Illumina Genome Analyzer (GA) II, on the same DNA sample obtained from a complex freshwater planktonic community. Despite the substantial differences… CONTINUE READING