Diphtheria toxin at low pH depolarizes the membrane, increases the membrane conductance and induces a new type of ion channel in Vero cells.

@article{Eriksen1994DiphtheriaTA,
  title={Diphtheria toxin at low pH depolarizes the membrane, increases the membrane conductance and induces a new type of ion channel in Vero cells.},
  author={Stuart Eriksen and Sjur Olsnes and Kirsten Sandvig and Ole Christian Sand},
  journal={The EMBO journal},
  year={1994},
  volume={13 19},
  pages={4433-9}
}
Receptor-dependent translocation of diphtheria toxin across the surface membrane of Vero cells was studied using patch clamp techniques. Translocation was induced by exposing cells with surface-bound toxin to low pH. Whole cell current and voltage clamp recordings showed that toxin translocation was associated with membrane depolarization and increased membrane conductance. The conductance increase was voltage independent, with a reversal potential of approximately 15 mV. This value was… CONTINUE READING