Dipeptidyl peptidase III from human erythrocytes.

  title={Dipeptidyl peptidase III from human erythrocytes.},
  author={Marija Abrami{\'c} and M Zubanovi{\'c} and Ljubinka Vitale},
  journal={Biological chemistry Hoppe-Seyler},
  volume={369 1},
Purification procedure for dipeptidyl peptidase III (DPP III) from human erythrocytes cytosol, entailing separations on DEAE-cellulose, hydroxylapatite and Sephacryl S-200 column, which gave homogeneous preparation in 35% yield, is described. The enzyme was shown to be a monomeric acidic protein (Mr approximately 82,000, pI approximately 4.5-4.6), sensitive to freezing and temperatures above 40 degrees C. It was inhibited by metallo-chelators and sulphydryl reagents, the activity being restored… CONTINUE READING