Dimerization of human 5-lipoxygenase.

  title={Dimerization of human 5-lipoxygenase.},
  author={A W H{\"a}fner and Mihaela Cernescu and Bettina Hofmann and Michael Ermisch and Michael Hoernig and Julia Metzner and Gisbert Schneider and Bernhard Brutschy and Dieter Steinhilber},
  journal={Biological chemistry},
  volume={392 12},
Human 5-lipoxygenase (5-LO) can form dimers as shown here via native gel electrophoresis, gel filtration chromatography and LILBID (laser induced liquid bead ion desorption) mass spectrometry. After glutathionylation of 5-LO by diamide/glutathione treatment, dimeric 5-LO was no longer detectable and 5-LO almost exclusively exists in the monomeric form which showed full catalytic activity. Incubation of 5-LO with diamide alone led to a disulfide-bridged dimer and to oligomer formation which… CONTINUE READING


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