Dil and DiO: versatile fluorescent dyes for neuronal labelling and pathway tracing

  title={Dil and DiO: versatile fluorescent dyes for neuronal labelling and pathway tracing},
  author={Marcia G. Honig and Richard I. Hume},
  journal={Trends in Neurosciences},
The fluorescent carbocyanine dyes dil and diO have an extensive history of use in cell biology, but their use as neuronal tracers is relatively recent. We found in 1985 that these molecules were excellent retrograde and anterograde tracers in the developing nervous system. We went on to show that these dyes were retained in neurons placed in culture, that they initially labelled the processes as well as the cell bodies of cultured neurons, and that they were seemingly non-toxic. We suggested… CONTINUE READING
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