Digital transcriptome profiling using selective hexamer priming for cDNA synthesis

  title={Digital transcriptome profiling using selective hexamer priming for cDNA synthesis},
  author={C. Armour and J. Castle and R. Chen and Tomas Babak and P. Loerch and Stuart G. Jackson and Jyoti K. Shah and John Dey and C. Rohl and J. Johnson and C. Raymond},
  journal={Nature Methods},
  • C. Armour, J. Castle, +8 authors C. Raymond
  • Published 2009
  • Biology, Medicine
  • Nature Methods
  • We developed a procedure for the preparation of whole transcriptome cDNA libraries depleted of ribosomal RNA from only 1 μg of total RNA. The method relies on a collection of short, computationally selected oligonucleotides, called 'not-so-random' (NSR) primers, to obtain full-length, strand-specific representation of nonribosomal RNA transcripts. In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing. 

    Figures, Tables, and Topics from this paper.

    RNA sequencing: advances, challenges and opportunities
    • 1,573
    • PDF
    Improving RNA-Seq expression estimates by correcting for fragment bias
    • 852
    • Highly Influenced
    • PDF
    Studying bacterial transcriptomes using RNA-seq
    • 228
    • PDF
    RNA‐Seq methods for transcriptome analysis
    • 199
    Digital transcriptome profiling from attomole-level RNA samples.
    • 62
    • Highly Influenced
    • PDF
    Efficient and robust RNA-seq process for cultured bacteria and complex community transcriptomes
    • 179


    Publications referenced by this paper.
    Alternative Isoform Regulation in Human Tissue Transcriptomes
    • 3,875
    • PDF
    Antisense Transcription in the Mammalian Transcriptome
    • 1,482