Digital transcriptome profiling using selective hexamer priming for cDNA synthesis

  title={Digital transcriptome profiling using selective hexamer priming for cDNA synthesis},
  author={C. Armour and J. Castle and R. Chen and Tomas Babak and P. Loerch and Stuart G. Jackson and Jyoti K. Shah and John Dey and C. Rohl and J. Johnson and C. Raymond},
  journal={Nature Methods},
  • C. Armour, J. Castle, +8 authors C. Raymond
  • Published 2009
  • Biology, Medicine
  • Nature Methods
  • We developed a procedure for the preparation of whole transcriptome cDNA libraries depleted of ribosomal RNA from only 1 μg of total RNA. The method relies on a collection of short, computationally selected oligonucleotides, called 'not-so-random' (NSR) primers, to obtain full-length, strand-specific representation of nonribosomal RNA transcripts. In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing. 
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