Digital separation of diaminobenzidine-stained tissues via an automatic color-filtering for immunohistochemical quantification.

@article{Fu2015DigitalSO,
  title={Digital separation of diaminobenzidine-stained tissues via an automatic color-filtering for immunohistochemical quantification.},
  author={Rong Fu and Xiaomian Ma and Zhaoying Bian and Jianhua Ma},
  journal={Biomedical optics express},
  year={2015},
  volume={6 2},
  pages={
          544-58
        }
}
The digital separation of diaminobenzidine (DAB)-stained tissues from hematoxylin background is an important pre-processing step to analyze immunostains. In most stain separation methods, specific color channels (for example: RGB, HSI, CMYK) or color deconvolution matrices are used to obtain different tissue contrasts between DAB- and hematoxylin-stained areas. However, these methods could produce incomplete separation or color changes because the color spectra of stains and co-localized stains… CONTINUE READING
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