Digital polymerase chain reaction for detecting c-MYC copy number gain in tissue and cell-free plasma samples of colorectal cancer patients

@inproceedings{Lee2018DigitalPC,
  title={Digital polymerase chain reaction for detecting c-MYC copy number gain in tissue and cell-free plasma samples of colorectal cancer patients},
  author={Kyu Sang Lee and Soo Kyung Nam and Soo Hyun Seo and Kyoung Un Park and Heung Kwon Oh and Duck-Woo Kim and Sung Bum Kang and Woo Ho Kim and Hye Seung Lee},
  booktitle={Scientific Reports},
  year={2018}
}
We focused on the utility of the droplet digital polymerase chain reaction (ddPCR) for detecting c-MYC gene copy number (GCN) gain in cell-free plasma and tumor tissue of colorectal cancer (CRC) patients. c-MYC GCN status was determined using dual-color silver in situ hybridization (SISH) and ddPCR in retrospective cohort 1 (192 CRC patients) and prospective cohort 2 (64 CRC patients). In cohort 1, c-MYC GCN gain was observed in 34 (17.5%) patients by SISH, and in 7 (3.6%) patients by ddPCR. c… CONTINUE READING
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