Digital imaging microscopy of firefly luciferase activity to directly monitor differences in cell transduction efficiencies between AdCMVLuc and Ad5LucRGD vectors having different cell binding properties.

@article{Kratzer2001DigitalIM,
  title={Digital imaging microscopy of firefly luciferase activity to directly monitor differences in cell transduction efficiencies between AdCMVLuc and Ad5LucRGD vectors having different cell binding properties.},
  author={Silvia Kratzer and Olaf Mundigl and Frank Dicker and Siegfried Seeber},
  journal={Journal of virological methods},
  year={2001},
  volume={93 1-2},
  pages={175-9}
}
The luciferase reporter gene incorporated into adenoviral vectors is very useful for monitoring viral transduction of different cell types or for comparing the transduction efficiency of different viral constructs of one cell type. Luciferase protein expression can be detected and quantified with very high sensitivity from whole cells or organ extracts. However, its disadvantages become obvious when aiming at evaluation of transduction events at the single cell level. The results obtained from… CONTINUE READING