Digital imaging in pathology: whole-slide imaging and beyond.

@article{Ghaznavi2013DigitalII,
  title={Digital imaging in pathology: whole-slide imaging and beyond.},
  author={Farzad Ghaznavi and Andrew Evans and Anant Madabhushi and Michael Feldman},
  journal={Annual review of pathology},
  year={2013},
  volume={8},
  pages={
          331-59
        }
}
Digital imaging in pathology has undergone an exponential period of growth and expansion catalyzed by changes in imaging hardware and gains in computational processing. Today, digitization of entire glass slides at near the optical resolution limits of light can occur in 60 s. Whole slides can be imaged in fluorescence or by use of multispectral imaging systems. Computational algorithms have been developed for cytometric analysis of cells and proteins in subcellular locations by use of… 

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References

SHOWING 1-10 OF 136 REFERENCES

Automated image analysis in histopathology: a valuable tool in medical diagnostics

TLDR
There are several image analysis software programs focusing on the quantification of immunohistochemical staining, particular attention is paid to this application in this review, and the market for automated image analysis tools will greatly expand over the next 10 years.

Digital microscopy: past, present, and future.

  • C. Hedvat
  • Medicine
    Archives of pathology & laboratory medicine
  • 2010
TLDR
The state of digital microscopy as it applies to the field of pathology is described and specific issues related to adoption of whole slide imaging systems are defined.

Primary histologic diagnosis using automated whole slide imaging: a validation study

TLDR
The results indicated that the image information contained in current whole slide images is sufficient for pathologists to make reliable diagnostic decisions and compose complex diagnostic reports, but this does not mean that WSI is as good as a microscope.

Multispectral imaging in biology and medicine: Slices of life

  • R. LevensonJ. Mansfield
  • Biology
    Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2006
TLDR
Examples derived from liquid‐crystal tunable filter‐based technology, which provide solutions to some of the major challenges in fluorescence‐based imaging, are presented to showcase the advantages of multispectral imaging in general.

Spectral imaging perspective on cytomics

  • R. Levenson
  • Biology
    Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2006
TLDR
Cytomics involves the analysis of cellular morphology and molecular phenotypes, with reference to tissue architecture and to additional metadata, as well as the collection of morphological and molecular information.

Standardizing the use of whole slide images in digital pathology

Implementation of whole slide imaging in surgical pathology: A value added approach

TLDR
A "value added" approach developed to guide decisions regarding integration of WSI into surgical pathology practice focuses on specific operational measures (cost, time, and enhanced patient care) and practice settings to identify routine activities in which the addition of W SI can provide improvements.

Spectral Imaging and Pathology: Seeing More

TLDR
There is little discernible enthusi-asm among practicing pathologists forobjective, quantitative image-based de-scriptors, at least for metrics such as nuclear grade or dysplasia, and spectral imaging facilitates the use and extension of molecular pathology approaches.
...