Digital autofocus methods for automated microscopy.

  title={Digital autofocus methods for automated microscopy.},
  author={Feimo Shen and Louis Hodgson and Klaus Michael Hahn},
  journal={Methods in enzymology},
Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with multifield scanning are detailed. 

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