Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer

@article{Morrissy2010DigitalGE,
  title={Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer},
  author={Sorana A. Morrissy and Yongjun Zhao and Allen D. Delaney and Jennifer Asano and Noreen Dhalla and Irene Li and Helen L McDonald and Pawan Pandoh and Anna-liisa Prabhu and Angela Tam and Martin Hirst and Marco A. Marra},
  journal={Current Protocols in Human Genetics},
  year={2010},
  volume={65}
}
This unit provides a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag‐seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing both the cost and time required to generate gene expression profiles. The ultra‐high‐throughput sequencing capability of the Illumina platform allows the cost‐effective generation of libraries containing an average of 20… Expand
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