Digital Encoding of Cellular mRNAs Enabling Precise and Absolute Gene Expression Measurement by Single-Molecule Counting

@inproceedings{Fu2014DigitalEO,
  title={Digital Encoding of Cellular mRNAs Enabling Precise
and Absolute Gene Expression Measurement by Single-Molecule Counting},
  author={Glenn K. Fu and Julie Wilhelmy and David Stern and H. Christina Fan and Stephen P. A. Fodor},
  booktitle={Analytical chemistry},
  year={2014}
}
We present a new approach for the sensitive detection and accurate quantitation of messenger ribonucleic acid (mRNA) gene transcripts in single cells. First, the entire population of mRNAs is encoded with molecular barcodes during reverse transcription. After amplification of the gene targets of interest, molecular barcodes are counted by sequencing or scored on a simple hybridization detector to reveal the number of molecules in the starting sample. Since absolute quantities are measured… CONTINUE READING
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Proceedings of the National Academy of Sciences of the United States of America

  • G K Fu, W Xu, +4 authors S P A Fodor
  • Proceedings of the National Academy of Sciences…
  • 2014

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