Digestive amylase from the larger grain borer, Prostephanus truncatus Horn.

@article{MendiolaOlaya2000DigestiveAF,
  title={Digestive amylase from the larger grain borer, Prostephanus truncatus Horn.},
  author={Elizabeth Mendiola-Olaya and Arnubio Valencia-Jim{\'e}nez and Silvia Vald{\'e}s-Rodr{\'i}guez and John Paul D{\'e}lano-Frier and Alejandro Blanco-Labra},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2000},
  volume={126 3},
  pages={425-33}
}
A combination of ion-exchange chromatography, preparative electrophoresis and gel filtration chromatography allowed a 1209-fold purification of one of the two major digestive alpha-amylases from larvae of the larger grain borer, Prostephanus truncatus Horn. The purified enzyme showed a molecular mass of 60.2 kDa, an isoelectric point of 4.7 and an optimal pH for activity of 6.0. The enzyme was heat labile and it was recognized by proteinaceous inhibitors from amaranth seeds (Amaranthus… CONTINUE READING

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