Digestion with matrix-bound proteases as a possible probe for the topography of the DNA-dependent RNA polymerase from Escherichia coli.

@article{Lill1975DigestionWM,
  title={Digestion with matrix-bound proteases as a possible probe for the topography of the DNA-dependent RNA polymerase from Escherichia coli.},
  author={Holger Lill and Guido R. Hartmann},
  journal={European journal of biochemistry},
  year={1975},
  volume={54 1},
  pages={45-53}
}
DNA-dependent RNA polymerase lacking subunit sigma was digested with matrix-bound chymotrypsin or trypsin in the presence of 0.4 M NaCl in the monomeric form or at low ionic strength in the oligomeric form. Sigma-containing polymerase was digested in the same way. The course of proteolysis was followed by polyacrylamide gel electrophoresis after… CONTINUE READING