Diffusion pseudotime robustly reconstructs lineage branching

  title={Diffusion pseudotime robustly reconstructs lineage branching},
  author={Laleh Haghverdi and M. B{\"u}ttner and F. A. Wolf and Florian Buettner and F. Theis},
  journal={Nature Methods},
  • Laleh Haghverdi, M. Büttner, +2 authors F. Theis
  • Published 2016
  • Biology, Medicine
  • Nature Methods
  • The temporal order of differentiating cells is intrinsically encoded in their single-cell expression profiles. We describe an efficient way to robustly estimate this order according to diffusion pseudotime (DPT), which measures transitions between cells using diffusion-like random walks. Our DPT software implementations make it possible to reconstruct the developmental progression of cells and identify transient or metastable states, branching decisions and differentiation endpoints. 
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