Diffusion of proteins across the nuclear envelope of HeLa cells.

@article{Chatterjee1998DiffusionOP,
  title={Diffusion of proteins across the nuclear envelope of HeLa cells.},
  author={Sandipan Chatterjee and Ursula Stochaj},
  journal={BioTechniques},
  year={1998},
  volume={24 4},
  pages={
          668-74
        }
}
We describe an experimental system to study nucleocytoplasmic diffusion of proteins in living HeLa cells. To localize proteins to the nucleus, substrates were created that contain a nuclear localization sequence fused to Aequorea victoria green fluorescent protein (GFP). Transiently and stably transfected HeLa cells were used for these assays. A protein of 29-kDa molecular mass that harbors GFP and the bipartite Xenopus nucleoplasmin nuclear localization sequence (NLS) accumulates efficiently… CONTINUE READING

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