Diffusion of macrolide antibiotics through the outer membrane of Moraxella catarrhalis

  title={Diffusion of macrolide antibiotics through the outer membrane of Moraxella catarrhalis},
  author={Hideto Tsujimoto and Naomasa Gotoh and Takeshi Nishino},
  journal={Journal of Infection and Chemotherapy},
We reported previously that the high susceptibility of Moraxella catarrhalis to macrolide antibiotics and other hydrophobic antimicrobial agents was related to the hydrophobicity of the cell surface. Electrophoretic analysis of lipopolysaccharide (LPS) extracted from M. catarrhalis revealed a deep rough-type profile similar to that of an LPS Re type mutant of Salmonella typhimurium, which also exhibits high susceptibility to macrolides. Moreover, treatment of 32P-labeled cells of M. catarrhalis… 
The structure of lipopolysaccharide transport protein B (LptB) from Burkholderia pseudomallei.
Highly conserved sequence and structural features are discussed that allow LptB to fuel the transport of lipid A in the thick outer membrane of Gram-negative bacteria.
A systematic quantitative proteomic examination of multidrug resistance in Acinetobacter baumannii.
The observed differences, combined with protein functional annotation, suggest that complex protein expression changes may accompany the MDR phenotype, and can potentially account for the observed resistance of BAA-1605 to 18 antibiotics.
Bacterial Polysaccharides: An Overview
Bacterial cell wall and membrane are associated with a variety of glycoconjugates and polysaccharides which aids in structural formation as well as performing various functions in the bacterial cell.
Alternative antimicrobials: the properties of fatty acids and monoglycerides
Assessment of the antimicrobial properties that have been demonstrated and the feasibility of therapeutic applications of antimicrobial fatty acids and their derivatives against a variety of bacterial species are reviewed.
Novel Polymyxin Combination With Antineoplastic Mitotane Improved the Bacterial Killing Against Polymyxin-Resistant Multidrug-Resistant Gram-Negative Pathogens
Results have important implications for repositioning non-antibiotic drugs for antimicrobial purposes, which may expedite the discovery of novel therapies to combat the rapid emergence of antibiotic resistance.
Antibacterial Activity of Polyoxometalates Against Moraxella catarrhalis
It is demonstrated, that POM activity is mainly depending on composition, shape, and size, but in the case of medium-size POTs (charge is more than −12 and number of addenda atoms is not being higher than 22) its activity correlates with the total net charge.
The Rho-Dependent Transcription Termination Is Involved in Broad-Spectrum Antibiotic Susceptibility in Escherichia coli
It is shown that E. coli with compromised Rho-dependent transcription termination function has enhanced broad-spectrum antibiotic susceptibility, which arises from the inefficient TolC-efflux process and increased permeability of the membrane.
Fatty acids and monoglycerides as novel prophylaxis against gonococcal ophthalmia neonatorum
There is potential in the use a fatty acid or fatty acid derivative, most likely monocaprin, to be used as the active antimicrobial agent in an ophthalmic prophylaxis but more evaluation in terms of in vivo testing is required to demonstrate that the higher levels of monOCaprin do not cause irritation to the eye.
Periplasmic mechano-transduction networks
Unlike the inner-membrane, the outer-membrane of Gram-negative bacteria cannot be energised by a proton gradient and the periplasmic space is absent of ATP. This poses a significant problem for
Novel Glycerophospholipid, Lipo- and N-acyl Amino Acids from Bacteroidetes: Isolation, Structure Elucidation and Bioactivity
The ‘core’ metabolome of the Bacteroidetes genus Chitinophaga was recently discovered to consist of only seven metabolites. A structural relationship in terms of shared lipid moieties among four of


Permeability of the outer membrane of Moraxella catarrhalis for beta-lactam antibiotics.
The susceptibility of ten clinical isolates and a standard reference strain, ATCC25238, of Moraxella catarrhalis to 22 beta-lactam antibiotics was examined and compared with that of Escherichia coli
Supersusceptibility to hydrophobic antimicrobial agents and cell surface hydrophobicity in Branhamella catarrhalis.
Its cell surface hydrophobicity was found to be markedly more hydrophobic than that of Escherichia coli or Pseudomonas aeruginosa cells, suggesting that the outer membrane of B. catarrhalis plays no role as a diffusion barrier towardshydrophobic agents.
An in-vitro comparison of the intraphagocytic bioactivity of erythromycin and roxithromycin.
Depletion of polymorph O2-dependent intrinsic antimicrobial systems interfered with the intracellular bioactivity of both antibiotics, emphasizing the importance of interactions between cell-associated antibiotics and phagocyte antimicrobialsystems in the elimination of intrACEllular microbial pathogens.
Comparative immunochemistry of lipopolysaccharides from Branhamella catarrhalis strains
Immunoblotting, passivehemolysis, and hemolysis inhibition assays using anti-LPS antibodies from immunized rabbits demonstrated cross-reactivity between the LPS preparations; however, antigenic dissimilarities were also found, suggesting that more than one serotype may exist.
An in-vitro evaluation of the cellular uptake and intraphagocytic bioactivity of clarithromycin (A-56268, TE-031), a new macrolide antimicrobial agent.
Clarithromycin is clearly a potent intraphagocytic antibiotic and potentially superior in this respect to erythromycin, and was found to possess intracellular bioactivity for all three species of bacteria with clarithromyzin being consistently more active than erystromycin.
Branhamella catarrhalis: an organism gaining respect as a pathogen.
  • B. Catlin
  • Medicine, Biology
    Clinical Microbiology Reviews
  • 1990
B. catarrhalis is currently recognized as the third most frequent cause of acute otitis media and acute sinusitis in young children and is incriminated occasionally in meningitis, endocarditis, bacteremia, conjunctivitis, keratitis, and urogenital infections.
Outer membranes of Gram‐negative bacteria are permeable to steroid probes
Study of various Gram‐negative species revealed that P. testosteroni, Pseudomonas acidovorans, and Acinetobacter calcoaceticus showed higher outer membrane permeability to steroid probes and higher susceptibility to hydrophobic agents such as fusidic acid, novobiocin and crystal violet relative to S. typhimurium and Escherichia coli.
Multidrug efflux pumps of gram-negative bacteria.
  • H. Nikaido
  • Medicine, Biology
    Journal of bacteriology
  • 1996
Recent studies showed that multipledrug efflux pumps, many with unusually broad specificities, play a major role in the intrinsic resistance of gram-negative bacteria.
Purification and characterization of macrolide 2'-phosphotransferase from a strain of Escherichia coli that is highly resistant to erythromycin
Macrolide 2'-phosphotransferase [MPH(2')] was purified 90-fold from an erythromycin-resistant strain of Escherichia coli, and its enzymatic properties were investigated. MPH(2') is an inducible
Agents that increase the permeability of the outer membrane.
  • M. Vaara
  • Biology, Medicine
    Microbiological reviews
  • 1992
Chelators (such as EDTA, nitrilotriacetic acid, and sodium hexametaphosphate), which disintegrate the outer membrane by removing Mg2+ and Ca2+, are effective and valuable permeabilizers.