Diffusion of fluorescently labeled macromolecules in cultured muscle cells.

@article{ArrioDupont1996DiffusionOF,
  title={Diffusion of fluorescently labeled macromolecules in cultured muscle cells.},
  author={M. Arrio-Dupont and Sophie Cribier and Georges Foucault and Philippe F. Devaux and Anne d'Albis},
  journal={Biophysical journal},
  year={1996},
  volume={70 5},
  pages={2327-32}
}
Myotubes were obtained from culture of satellite cells. They had a sarcomeric organization similar to that of muscle. The diffusion in the direction perpendicular to the fibers of microinjected fluorescein isothiocyanate-dextrans of molecular weight ranging from 9500 to 150,000 was examined by modulated fringe pattern photobleaching. On the time scale of the observation, 10-30 S, all of the dextrans were completely mobile in the cytoplasm. The diffusion coefficients were compared to the values… CONTINUE READING

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