Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support.

@article{Olsen1998DiffusionBF,
  title={Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support.},
  author={Ingrid Olsen and Harald G. Wiker},
  journal={Methods in molecular biology},
  year={1998},
  volume={536},
  pages={
          35-8
        }
}
A very simple and fast method for diffusion blotting of proteins from precast SDS-PAGE gels on a solid plastic support was developed. Diffusion blotting for 3 min gave a quantitative transfer of 10% compared with 1-h electroblotting. For each subsequent blot from the same gel a doubling of transfer time is necessary to obtain the same amount of protein onto each blot. The relative transfer of low and high molecular weight components was similar in diffusion and electroblotting. However, both… CONTINUE READING