Diffusion and directed movement: in vitro motile properties of fission yeast kinesin-14 Pkl1.

@article{Furuta2008DiffusionAD,
  title={Diffusion and directed movement: in vitro motile properties of fission yeast kinesin-14 Pkl1.},
  author={Ken'ya Furuta and Masaki Edamatsu and Yurina Maeda and Yoko Yano Toyoshima},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 52},
  pages={36465-73}
}
Fission yeast Pkl1 is a kinesin-14A family member that is known to be localized at the cellular spindle and is capable of hydrolyzing ATP. However, its motility has not been detected. Here, we show that Pkl1 is a slow, minus end-directed microtubule motor with a maximum velocity of 33+/-9 nm/s. The Km,MT value of steady-state ATPase activity of Pkl1 was as low as 6.4+/-1.1 nM, which is 20-30 times smaller than that of kinesin-1 and another kinesin-14A family member, Ncd, indicating a high… CONTINUE READING

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A nrew s on M arch 4, 2015 hp://w w w .jb.org/ D

A. Lupas
Motility of Fission Yeast Pkl1 DECEMBER • 1996
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