Diffusion-Limited Cargo Loading of an Engineered Protein Container.

@article{Zschoche2015DiffusionLimitedCL,
  title={Diffusion-Limited Cargo Loading of an Engineered Protein Container.},
  author={Reinhard Matthias Zschoche and Donald Hilvert},
  journal={Journal of the American Chemical Society},
  year={2015},
  volume={137 51},
  pages={
          16121-32
        }
}
The engineered bacterial nanocompartment AaLS-13 is a promising artificial encapsulation system that exploits electrostatic interactions for cargo loading. In order to study its ability to take up and retain guests, a pair of fluorescent proteins was developed which allows spectroscopic determination of the extent of encapsulation by Förster resonance energy transfer (FRET). The encapsulation process is generally complete within a second, suggesting low energetic barriers for proteins to cross… CONTINUE READING
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