Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking

  title={Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking},
  author={Maxime Dahan and Sabine L{\'e}vi and Camilla Luccardini and Philippe Rostaing and B{\'e}atrice Riveau and Antoine Triller},
  pages={442 - 445}
Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further… 
Labeling neuronal membrane receptors with quantum dots.
This protocol describes a highly sensitive approach for tracking the motion of membrane molecules over extended time periods with single-molecule resolution. This technique uses nanometer-sized
Real-time quantum dot tracking of single proteins.
In this chapter, a basic introduction and experimental setup for single quantum dot labeling of a target protein is given and data acquisition and analysis of time-lapse single quantumdot imaging with sample protocols are provided.
Stable small quantum dots for synaptic receptor tracking on live neurons.
A coating method to produce functionalized small quantum dots (sQDs) that were stable for over a month indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.
Single quantum dot tracking of membrane receptors.
The experimental protocols and methods of analysis used to investigate the dynamics of individual GABAA receptors in the axonal growth cone of spinal neurons in culture are presented, finding them suitable to study many transmembrane proteins.
Antibody-Conjugated Single Quantum Dot Tracking of Membrane Neurotransmitter Transporters in Primary Neuronal Cultures.
This chapter describes a generalizable protocol for the single particle tracking of membrane neurotransmitter transporters on cell membranes with either unmodified extracellular antibody probes and secondary antibody-conjugated quantum dots or biotinylatedextracellular antibodies and streptavidin-conjoined quantum dots in primary neuronal cultures.
Tracking individual proteins in living cells using single quantum dot imaging.
This chapter presents methods used for tracking single biomolecules coupled to quantum dots in living cells from labeling procedures to the analysis of the quantum dot motion.
Imaging single synaptic vesicles in Mammalian central synapses with quantum dots.
  • Qi Zhang
  • Materials Science, Medicine
    Methods in molecular biology
  • 2013
This protocol describes a sensitive and rigorous method to monitor the movement and turnover of single synaptic vesicles in live presynaptic terminals of mammalian central nervous system. This
Imaging the lateral diffusion of membrane molecules with quantum dots
This protocol describes a sensitive approach to tracking the motion of membrane molecules such as lipids and proteins with molecular resolution in live cells. This technique makes use of fluorescent
A multiple target approach for single quantum dot tracking
  • Stephane Bonneau, L. Cohen, M. Dahan
  • Physics, Computer Science
    2004 2nd IEEE International Symposium on Biomedical Imaging: Nano to Macro (IEEE Cat No. 04EX821)
  • 2004
An algorithm for automatically detecting and tracking QDs in sequences of fluorescence images is presented, which accounts for the blinking in the fluorescence signal of individual QDs assuming Brownian dynamics of the biomolecules.
Single Quantum Dot Tracking Illuminates Neuroscience at the Nanoscale.
The evolution of single quantum dot tracking over the past two decades is reviewed, key biophysical discoveries facilitated by quantum dots are highlighted, and recent accomplishments at the interface of molecular neuroscience and nanoscience are reported.


Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
This work characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals and found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.
Fast and reversible trapping of surface glycine receptors by gephyrin
Data show that receptor number in a cluster results from a dynamic equilibrium between the pools of stabilized and freely mobile receptors, which could be involved in regulation of the number of receptors at synapses.
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
C encapsulated individual nanocrystals in phospholipid block–copolymer micelles acted as in vitro fluorescent probes to hybridize to specific complementary sequences and were followed to the tadpole stage, allowing lineage-tracing experiments in embryogenesis.
Luminescent quantum dots for multiplexed biological detection and imaging.
This work has shown how the emission wavelength of quantum-dot nanocrystals can be continuously tuned by changing the particle size, and a single light source can be used for simultaneous excitation of all different-sized dots.
Direct imaging of lateral movements of AMPA receptors inside synapses
The data show that rapid exchange of receptors between a synaptic and extra‐synaptic localization occurs through regulation of receptor diffusion inside synapses, and that this process is driven by inhibition of inhibitory transmission to favor excitatory synaptic activity.
Autofluorescent proteins in single-molecule research: applications to live cell imaging microscopy.
The yellow-fluorescent protein mutant eYFP is superior compared to all the fluorescent proteins for single-molecule studies in vivo and is found to dynamically track the entity to which it is anchored when bound to membranes of live cells.
Fluorescence intermittency in single cadmium selenide nanocrystals
SEMICONDUCTOR nanocrystals offer the opportunity to study the evolution of bulk materials properties as the size of a system increases from the molecular scale1,2. In addition, their strongly
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Using QDs linked to immunoglobulin G and streptavidin to label the breast cancer marker Her2, to stain actin and microtubule fibers in the cytoplasm, and to detect nuclear antigens inside the nucleus indicate that QD-based probes can be very effective in cellular imaging and offer substantial advantages over organic dyes in multiplex target detection.
Strychnine-Blocked Glycine Receptor Is Removed from Synapses by a Shift in Insertion/Degradation Equilibrium
Results indicate that strychnine does not destabilize the postsynaptic receptor but triggers its disappearance from synapses by a nondegradative sequestration of newly synthesized molecules in a nonendocytic compartment.
Single-Molecule Analysis of Chemotactic Signaling in Dictyostelium Cells
The dynamic properties of receptors involved in gradient sensing are illustrated and it is suggested that these may be polarized in chemotactic cells.