Diffusible and bound actin in nuclei of xenopus laevis oocytes

@article{Clark1977DiffusibleAB,
  title={Diffusible and bound actin in nuclei of xenopus laevis oocytes},
  author={T. Clark and R. Merriam},
  journal={Cell},
  year={1977},
  volume={12},
  pages={883-891}
}
Several criteria have been used to identify actin in hand-isolated nuclei of Xenopus laevis oocytes; these include co-migration with actin on SDS-polyacrylamide gels, immunological cross-reactivity with antiserum against actin, binding to DNAase I and peptide mapping on SDS gels. The use of hand-isolated nuclei precludes the possibility of contamination from cytoplasmic actin or the leakage of significant amounts of actin from nuclei during isolation. Actin constitutes roughly 6% of the total… Expand
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