Differentiation of vaccine and wild mumps viruses using the polymerase chain reaction and dideoxynucleotide sequencing.

@article{Forsey1990DifferentiationOV,
  title={Differentiation of vaccine and wild mumps viruses using the polymerase chain reaction and dideoxynucleotide sequencing.},
  author={Timothy Forsey and Jennifer Mawn and Philip J Yates and Maureen L Bentley and Philip D. Minor},
  journal={The Journal of general virology},
  year={1990},
  volume={71 ( Pt 4)},
  pages={987-90}
}
Parts of the F gene from 16 mumps viruses derived from vaccines and clinical isolates were amplified using the polymerase chain reaction and their nucleotide sequences were determined. Over a region of 111 nucleotides, eight regions of variability were detected with a maximum of six (5.4%) changes occurring between any two virus strains. The Jeryl Lynn and Urabe vaccine strains were clearly different from each other and from wild virus isolated from cases of non-vaccine-associated mumps. In… CONTINUE READING