Differentiation of spinal motor neurons from pluripotent human stem cells

  title={Differentiation of spinal motor neurons from pluripotent human stem cells},
  author={Baoyang Hu and Su-chun Zhang},
  journal={Nature Protocols},
We have devised a reproducible protocol by which human embryonic stem cells (hESCs) or inducible pluripotent stem cells (iPSCs) are efficiently differentiated to functional spinal motor neurons. This protocol comprises four major steps. Pluripotent stem cells are induced to form neuroepithelial (NE) cells that form neural tube-like rosettes in the absence of morphogens in the first 2 weeks. The NE cells are then specified to OLIG2-expressing motoneuron progenitors in the presence of retinoic… CONTINUE READING
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