Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy

  title={Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy},
  author={J{\'e}rome Chal and Masayuki Oginuma and Ziad Al Tanoury and B{\'e}n{\'e}dicte Gobert and Olga Sumara and Aurore Hick and Fanny Bousson and Yasmine Zidouni and Caroline Mursch and Philippe Moncuquet and Olivier Tassy and St{\'e}phane D. Vincent and Ayako Miyanari and Agata N Bera and Jean Marie Garnier and Getzabel Guevara and Marie Hestin and Leif Kennedy and Shinichiro Hayashi and Bernadette Drayton and Thomas Cherrier and Barbara Gayraud-Morel and Emanuela Gussoni and Fr{\'e}d{\'e}ric Relaix and Shahragim Tajbakhsh and Olivier Pourqui{\'e}},
  journal={Nature Biotechnology},
During embryonic development, skeletal muscles arise from somites, which derive from the presomitic mesoderm (PSM). Using PSM development as a guide, we establish conditions for the differentiation of monolayer cultures of mouse embryonic stem (ES) cells into PSM-like cells without the introduction of transgenes or cell sorting. We show that primary and secondary skeletal myogenesis can be recapitulated in vitro from the PSM-like cells, providing an efficient, serum-free protocol for the… 

Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro

An optimized serum-free differentiation protocol to efficiently produce striated, millimeter-long muscle fibers together with satellite-like cells from human pluripotent stem cells (hPSCs) in vitro by mimicking key signaling events leading to muscle formation in the embryo is reported.

Induced Fetal Human Muscle Stem Cells with High Therapeutic Potential in a Mouse Muscular Dystrophy Model

Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro

It is demonstrated that cells exhibiting characteristics of human fetal satellite cells can be produced in vitro from iPSC, opening interesting avenues for muscular dystrophy cell therapy and providing significant insights into the development of the human myogenic lineage.

The Long Road to Making Muscle In Vitro.

Efficient Generation of Skeletal Myogenic Progenitors from Human Pluripotent Stem Cells

The following protocol will enable researchers to derive skeletal myogenic progenitors from pluripotent stem cells for disease modeling as wells as therapeutic purposes.

From pluripotency to myogenesis: a multistep process in the dish

This review summarizes the current state of the art, comparing spontaneous and directed myogenic differentiation of PSCs as well as the protocols developed this far to facilitate this process.

Human iPS Cells Derived Skeletal Muscle Progenitor Cells Promote Myoangiogenesis and Restore Dystrophin in Duchenne Muscular Dystrophic Mice

It is concluded that hiPSCs pharmacologically reprogrammed into MPC with a small molecule, Givi with anti-oxidative, anti-inflammatory and muscle gene promoting properties might be an effective cellular source for treatment of muscle injury and restoration of dystrophin in DMD.

Recapitulating early development of mouse musculoskeletal precursors of the paraxial mesoderm in vitro

Pluripotent stem cells are used to generate early paraxial mesoderm and subsequently to recapitulate the stages of musculoskeletal progenitor specification and differentiation into muscle.

Directed Differentiation of Human Pluripotent Stem Cells toward Skeletal Myogenic Progenitors and Their Purification Using Surface Markers

The current protocol describes a detailed, step-by-step guide for this improved directed differentiation method for the derivation of skeletal myogenic progenitors from hiPSCs and outlines important experimental details and troubleshooting points for its application in any human pluripotent stem cells.

Skeletal Muscle Cell Induction from Pluripotent Stem Cells

The current methods used in myogenic differentiation utilize techniques including overexpression of myogenic transcription factors such as MyoD or Pax3, using small molecules to induce mesodermal cells followed by myogenic progenitor cells, and utilizing epigenetic myogenic memory existing in muscle cell-derived iPSCs.



Directed In Vitro Myogenesis of Human Embryonic Stem Cells and Their In Vivo Engraftment

It is reported that hESCs can differentiate into skeletal muscle cells without genetic manipulation through the isolation of cells expressing a mesodermal marker, platelet-derived growth factor receptor-α (PDGFRA), following embryoid body (EB) formation.

Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells

  • Yuta MizunoHsi Chang T. Heike
  • Biology
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2010
It is demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells.

Myogenic Differentiation of Muscular Dystrophy‐Specific Induced Pluripotent Stem Cells for Use in Drug Discovery

It is demonstrated that iPSCs have no intrinsic barriers preventing MyoD from inducing efficient and rapid myogenesis and thus providing a scalable source of normal and dystrophic myoblasts for use in disease modeling and drug discovery.

Efficient derivation of lateral plate and paraxial mesoderm subtypes from human embryonic stem cells through GSKi-mediated differentiation.

This study presents a unique approach for generating early mesoderm progenitors in a chemically directed fashion through the use of small-molecule GSK-3 inhibitor, which may be useful for future applications in regenerative medicine.

Derivation of Myogenic Progenitors Directly From Human Pluripotent Stem Cells Using a Sphere‐Based Culture

A novel protocol for deriving myogenic progenitors from human embryonic stem and induced pluripotent stem cells using free‐floating spherical culture (EZ spheres) in a defined culture medium is presented.

In Vitro Modeling of Paraxial Mesodermal Progenitors Derived from Induced Pluripotent Stem Cells

A protocol for the differentiation of mouse iPS cells into paraxial mesodermal lineages in serum-free culture was established and exhibited differentiation potential into osteogenic, chondrogenic, and myogenic cells both in vitro and in vivo and contributed to muscle regeneration.

Paraxial Mesodermal Progenitors Derived from Mouse Embryonic Stem Cells Contribute to Muscle Regeneration via Differentiation into Muscle Satellite Cells

The results show the potential of ES cell‐derived paraxial mesodermal progenitor cells to generate functional muscle stem cells in vivo without inducing or suppressing gene manipulation to form the foundation of the development of stem cell therapies to repair diseased and damaged muscles.