Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy

@article{Chal2015DifferentiationOP,
  title={Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy},
  author={J. Chal and Masayuki Oginuma and Z. Al Tanoury and B. Gobert and O. Sumara and Aurore Hick and F. Bousson and Yasmine Zidouni and Caroline Mursch and P. Moncuquet and Olivier Tassy and S. Vincent and Ayako Miyanari and A. Bera and J. Garnier and Getzabel Guevara and M. Hestin and L. Kennedy and S. Hayashi and B. Drayton and T. Cherrier and Barbara Gayraud-Morel and E. Gussoni and F. Relaix and S. Tajbakhsh and O. Pourqui{\'e}},
  journal={Nature Biotechnology},
  year={2015},
  volume={33},
  pages={962-969}
}
During embryonic development, skeletal muscles arise from somites, which derive from the presomitic mesoderm (PSM). Using PSM development as a guide, we establish conditions for the differentiation of monolayer cultures of mouse embryonic stem (ES) cells into PSM-like cells without the introduction of transgenes or cell sorting. We show that primary and secondary skeletal myogenesis can be recapitulated in vitro from the PSM-like cells, providing an efficient, serum-free protocol for the… Expand
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