Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy

  title={Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy},
  author={J. Chal and Masayuki Oginuma and Z. Al Tanoury and B{\'e}n{\'e}dicte Gobert and O. Sumara and Aurore Hick and F. Bousson and Yasmine Zidouni and Caroline Mursch and Philippe Moncuquet and Olivier Tassy and S. Vincent and Ayako Miyanari and A. Bera and J. Garnier and Getzabel Guevara and M. Hestin and L. Kennedy and S. Hayashi and B. Drayton and T. Cherrier and Barbara Gayraud-Morel and E. Gussoni and F. Relaix and S. Tajbakhsh and Olivier Pourquié},
  journal={Nature Biotechnology},
  • J. Chal, Masayuki Oginuma, +23 authors Olivier Pourquié
  • Published 2015
  • Biology, Medicine
  • Nature Biotechnology
  • During embryonic development, skeletal muscles arise from somites, which derive from the presomitic mesoderm (PSM). Using PSM development as a guide, we establish conditions for the differentiation of monolayer cultures of mouse embryonic stem (ES) cells into PSM-like cells without the introduction of transgenes or cell sorting. We show that primary and secondary skeletal myogenesis can be recapitulated in vitro from the PSM-like cells, providing an efficient, serum-free protocol for the… CONTINUE READING
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