Differentiation of industrial wine yeast strains using microsatellite markers

  title={Differentiation of industrial wine yeast strains using microsatellite markers},
  author={A Gonz{\'a}lez Techera and Sandra Jubany and Francisco Carrau and Carina Gaggero},
  journal={Letters in Applied Microbiology},
Aims: To differentiate nine industrial wine strains of Saccharomyces cerevisiae using microsatellite (simple sequence repeats, SSR) markers. 

Microsatellite PCR profiling of Saccharomyces cerevisiae strains during wine fermentation

The use of microsatellite PCR to monitor populations of Saccharomyces cerevisiae strains during fermentation of grape juice will help improve the quality of research into grape juice spoilage and reduce the risks of spoilage.

Commercial yeast strains do not disseminate significantly in winery ecosystems of wine producing regions in France and Portugal

Apresentacao efectuada na Reuniao Geral da " Organisation International de la Vigne et du Vin", 83, Paris, 2003.

Microsatellite marker‐based assessment of the biodiversity of native bioethanol yeast strains

The high yeast diversity observed among native S. cerevisiae strains in various Brazilian sugar mills provides new insights on the use of autochthonous high‐fitness strains with industrial characteristics as starter cultures at bioethanol plants.


In winemaking, natural grape juice fermentation is carried out by a succession of different yeast populations, but in later stages of fermentation may appear the spoilage yeast Brettanomyces bruxellensis and other yeasts such as genera Pichia, Kluyveromyces, Candida or Hanseniaspora.

SSR-Marker Analysis—A Method for S. cerevisiae Strain Characterization and Its Application for Wineries

A method based on simple sequence repeats (SSRs) markers, occurring in the yeast genome, was developed to differentiate the collected S. cerevisiae strains and can be used for the preservation of the yeast diversity associated with given terroirs and as an option for an increased safety of fermentations.

Metabolic engineering of wine yeast and advances in yeast selection methods for improved wine quality and safety.

Most alcoholic fermentations in winemaking are nowadays performed by commercially produced yeast strains that are inoculated into the fermentation vessel, which provides new insights and opportunities for the generation of new yeast strains while holistically improving the knowledge of wine microbial ecology.

Use of interdelta polymorphisms of Saccharomyces cerevisiae strains to monitor population evolution during wine fermentation

It is shown that PCR-amplification of DNA delta sequences of S. cerevisiae strains is a reproducible, strain-specific and simple method that can be used successfully to monitor yeast strain population dynamics during wine fermentations.



PCR and DNA Fingerprinting Used as Quality Control in the Production of Wine Yeast Strains

DNA fingerprinting and polymerase chain reaction as identification tools were tested for their reproducibility and their use to study the genetic stability of more than a hundred wine yeast strains during standard industrial propagation conditions.

A Comparative Study of Different Methods of Yeast Strain Characterization

Differentiation and species identification of yeasts using PCR.

A PCR-based method has been developed that permits both intraspecies differentiation and species identification of yeast isolates, enabling the rapid grouping of isolates with related genomes and the investigation of population dynamics of strains of the same species.

Wine Yeasts Strain Idenification

A series of tests to identify and characterize yeast strains including rapid biochemical methods, killer profile, protein electrophoresis profile, volatile fatty acids profile, chromosome electrophoreis, and DNA fingerprinting are developed.

AFLP fingerprinting for analysis of yeast genetic variation.

Results from two species illustrate that AFLP is useful for the study of intraspecific genetic relatedness and demonstrates the potential of AFLP in yeast ecology and evolutionary studies.

Microsatellite polymorphism in the promoter sequence of the elongation factor 3 gene of Candida albicans as the basis for a typing system

The polymorphism of a TTC/TTTC microsatellite in the promoter sequence of the elongation factor 3 gene of Candida albicans was investigated by PCR, and reproducibility was achieved by automated procedures.

Historical Genetics: The Parentage of Chardonnay, Gamay, and Other Wine Grapes of Northeastern France.

Sixteen wine grapes that have long been grown in northeastern France, including 'Chardonnay', 'Gamay noir', 'Aligoté', and 'Melon', have microsatellite genotypes consistent with their being the progeny of a single pair of parents, 'Pinot' and 'Gouais blanc', both of which were widespread in this region in the Middle Ages.


The results suggest that the fermentation characteristics are specific for S. bayanus, which showed good fermentability at low temperatures accompanied by production of low amounts of ethanol at intermediate temperatures, the same as cryophilic wine yeasts.

The evolutionary dynamics of repetitive DNA in eukaryotes

Features of the organization of repetitive sequences in eukaryotic genomes, and their distribution in natural populations, reflect the evolutionary forces acting on selfish DNA.

Abundant microsatellite polymorphism in Saccharomyces cerevisiae, and the different distributions of microsatellites in eight prokaryotes and S. cerevisiae, result from strong mutation pressures and a variety of selective forces.

  • D. FieldC. Wills
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1998
In all these genomes there is an excess of repeats shorter than those traditionally considered to be microsatellites, suggesting that even in prokaryotes these repeats are being generated by mutational pressures, which has important potential implications for understanding genome stability and evolution in these microbial species.