Differentiation of bacterial autolysins by zymogram analysis.

  title={Differentiation of bacterial autolysins by zymogram analysis.},
  author={Hendrik Strating and Anthony J Clarke},
  journal={Analytical biochemistry},
  volume={291 1},
The use of zymograms in which the bacterial cell wall heteropolymer peptidoglycan is incorporated into the resolving gel of SDS-PAGE has led to the identification of various SDS stable peptidoglycan hydrolases (autolysins). To examine the specificity of autolysins with respect to O-acetylated peptidoglycan, a discontinuous SDS-PAGE system has been developed that operates under neutral conditions. [Bis(2-hydroxyethyl)imino]tris(hydroxymethyl)methane (Bis-Tris) buffers are employed with pH 6.8… CONTINUE READING
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