Differentiation of Cucumber Mosaic Virus Subgroups by Rt-pcr Rflp

  title={Differentiation of Cucumber Mosaic Virus Subgroups by Rt-pcr Rflp},
  author={M. M. Finetti Sialer and Fabrizio Cillo and Luigi Barbarossa and Donato Gallitelli},
A new cucumber mosaic virus (CMV) subgroup based on recent sequence data has been proposed by Palukaitis and Zaitlin (1997) to distinguish between some strains in the former subgroup I. The three subgroups are tentatively named IA, IB and II. Here we describe a simple and rapid procedure based on the reverse transcriptase-polymerase chain reaction (RTPCR) followed by enzymatic digestion (RFLP) to identify and classify CMV isolates accurately into the three subgroups. Two specific primers that… CONTINUE READING


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