Differentiation of Chlamydia species by combined use of polymerase chain reaction and restriction endonuclease analysis.

@article{Yoshida1998DifferentiationOC,
  title={Differentiation of Chlamydia species by combined use of polymerase chain reaction and restriction endonuclease analysis.},
  author={Hiromu Yoshida and Yuuki Kishi and Sadashi Shiga and Tomoko Hagiwara},
  journal={Microbiology and immunology},
  year={1998},
  volume={42 5},
  pages={411-4}
}
To differentiate Chlamydia spp., a primer pair designed to generate a genus-specific region of the major outer membrane protein (MOMP) gene was used in a PCR to amplify a single DNA fragment of 245-259 bp. In the PCR, the expected single DNA fragment was amplified from strains of Chlamydia trachomatis, C. psittaci, C. pneumoniae and C. pecorum, respectively. By restriction endonuclease analysis with AluI and PvuII, the amplified products exhibited four distinct patterns, corresponding to the… CONTINUE READING