Differentiation of Asian rice gall midge, Orseolia oryzae (Wood-Mason), biotypes by sequence characterized amplified regions (SCARs).

@article{Behura1999DifferentiationOA,
  title={Differentiation of Asian rice gall midge, Orseolia oryzae (Wood-Mason), biotypes by sequence characterized amplified regions (SCARs).},
  author={Sasmita K. Behura and Sarat Chandra Sahu and Sathish Rajamani and A. Senorita Devi and Rohit Mago and S Pradeep Nair and Madan Mohan},
  journal={Insect molecular biology},
  year={1999},
  volume={8 3},
  pages={391-7}
}
We developed a polymerase chain reaction (PCR)-based assay that distinguished five different biotypes of the Asian gall midge (Orseolia oryzae), a major insect pest of rice. A total of 400 random primers were screened using random amplified polymorphic DNAs (RAPDs). Five diagnostic PCR products were isolated, cloned, sequenced and converted to sequence characterized amplified regions (SCARs). Primers specific to these SCARs were able to amplify specific DNA fragments from genomic DNAs of five… CONTINUE READING
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