Differentiation between types and strains of Clostridium botulinum by riboprinting.

  title={Differentiation between types and strains of Clostridium botulinum by riboprinting.},
  author={Geoffrey E. Skinner and Steven M. Gendel and G A Fingerhut and Henry A Solomon and Jodie Ulaszek},
  journal={Journal of food protection},
  volume={63 10},
The ability of automated ribotyping to differentiate between major types and individual strains of Clostridium botulinum was tested using the Qualicon Riboprinter Microbial Characterization System. Pure spores of C. botulinum type A, proteolytic type B, nonproteolytic type B, and type E strains were inoculated onto modified anaerobic egg yolk agar and incubated 24 h at 35 degrees C. Plates were rinsed with buffer (2 mM Tris + 20 mM EDTA) to remove vegetative cells that were heated for 10 min at… CONTINUE READING

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